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rabbit anti p63 polyclonal  (Proteintech)


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    Structured Review

    Proteintech rabbit anti p63 polyclonal
    Fig. 3. (A) Agarose gel electropherogram of amplification products (passage 1). Lane 1: DNA ladder (M), lanes 2–3: KRT3 (101 bp), lanes 4–5: KRT12 (190 bp), lane 6: DNA ladder (M), lanes 7–8: TP63 (96 bp), lanes 9–10: ABCG2 (144 bp), lanes 11–12: ACTβ (131 bp), lane 13: DNA ladder (M). NC, negative control. (B) Visualization of lack of expression of CK3, and CK12 and expression of positive markers <t>p63</t> and ABCG2, in LSCs cultured in a medium supplemented with H/I/E. FITC-conjugated specific antibodies (green); DAPI- stained cell nuclei (blue). IC, isotype control. Scale bars = 15 μm.
    Rabbit Anti P63 Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p63+polyclonal/p63+Antibody/pm40210649-99-26-31
    Average 95 stars, based on 62 article reviews
    rabbit anti p63 polyclonal - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells."

    Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells.

    Journal: Scientific reports

    doi: 10.1038/s41598-025-96919-2

    Fig. 3. (A) Agarose gel electropherogram of amplification products (passage 1). Lane 1: DNA ladder (M), lanes 2–3: KRT3 (101 bp), lanes 4–5: KRT12 (190 bp), lane 6: DNA ladder (M), lanes 7–8: TP63 (96 bp), lanes 9–10: ABCG2 (144 bp), lanes 11–12: ACTβ (131 bp), lane 13: DNA ladder (M). NC, negative control. (B) Visualization of lack of expression of CK3, and CK12 and expression of positive markers p63 and ABCG2, in LSCs cultured in a medium supplemented with H/I/E. FITC-conjugated specific antibodies (green); DAPI- stained cell nuclei (blue). IC, isotype control. Scale bars = 15 μm.
    Figure Legend Snippet: Fig. 3. (A) Agarose gel electropherogram of amplification products (passage 1). Lane 1: DNA ladder (M), lanes 2–3: KRT3 (101 bp), lanes 4–5: KRT12 (190 bp), lane 6: DNA ladder (M), lanes 7–8: TP63 (96 bp), lanes 9–10: ABCG2 (144 bp), lanes 11–12: ACTβ (131 bp), lane 13: DNA ladder (M). NC, negative control. (B) Visualization of lack of expression of CK3, and CK12 and expression of positive markers p63 and ABCG2, in LSCs cultured in a medium supplemented with H/I/E. FITC-conjugated specific antibodies (green); DAPI- stained cell nuclei (blue). IC, isotype control. Scale bars = 15 μm.

    Techniques Used: Agarose Gel Electrophoresis, Amplification, Negative Control, Expressing, Cell Culture, Staining, Control

    Related Articles

    Incubation:

    Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells
    Article Snippet: LSCs after first passage were cultured on cover slips, fixed with 4% formaldehyde, and permeabilized with 0.5% Triton X-100 solution (Sigma-Aldrich, USA) and Tween 20 (Sigma-Aldrich, USA). .. After rinsing with PBS, the cells were incubated with blocking buffer for 1 h, rinsed again, and labelled with rabbit anti-ABCG2 polyclonal (1:50; 27286-AP; Proteintech, USA), rabbit anti-p63 polyclonal (1:50; 12143-1-AP; Proteintech, USA), mouse anti-CK3 monoclonal (1:50; ab68260; Abcam, UK), and rabbit anti-CK12 monoclonal (1:50; ab185627; Abcam, UK) primary antibodies at 4 °C overnight. ..

    Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells.
    Article Snippet: LSCs after first passage were cultured on cover slips, fixed with 4% formaldehyde, and permeabilized with 0.5% Triton X-100 solution (Sigma-Aldrich, USA) and Tween 20 (Sigma-Aldrich, USA). .. After rinsing with PBS, the cells were incubated with blocking buffer for 1 h, rinsed again, and labelled with rabbit anti-ABCG2 polyclonal (1:50; 27286-AP; Proteintech, USA), rabbit anti-p63 polyclonal (1:50; 12143-1-AP; Proteintech, USA), mouse anti-CK3 monoclonal (1:50; ab68260; Abcam, UK), and rabbit anti-CK12 monoclonal (1:50; ab185627; Abcam, UK) primary antibodies at 4 °C overnight. ..

    Blocking Assay:

    Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells
    Article Snippet: LSCs after first passage were cultured on cover slips, fixed with 4% formaldehyde, and permeabilized with 0.5% Triton X-100 solution (Sigma-Aldrich, USA) and Tween 20 (Sigma-Aldrich, USA). .. After rinsing with PBS, the cells were incubated with blocking buffer for 1 h, rinsed again, and labelled with rabbit anti-ABCG2 polyclonal (1:50; 27286-AP; Proteintech, USA), rabbit anti-p63 polyclonal (1:50; 12143-1-AP; Proteintech, USA), mouse anti-CK3 monoclonal (1:50; ab68260; Abcam, UK), and rabbit anti-CK12 monoclonal (1:50; ab185627; Abcam, UK) primary antibodies at 4 °C overnight. ..

    Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells.
    Article Snippet: LSCs after first passage were cultured on cover slips, fixed with 4% formaldehyde, and permeabilized with 0.5% Triton X-100 solution (Sigma-Aldrich, USA) and Tween 20 (Sigma-Aldrich, USA). .. After rinsing with PBS, the cells were incubated with blocking buffer for 1 h, rinsed again, and labelled with rabbit anti-ABCG2 polyclonal (1:50; 27286-AP; Proteintech, USA), rabbit anti-p63 polyclonal (1:50; 12143-1-AP; Proteintech, USA), mouse anti-CK3 monoclonal (1:50; ab68260; Abcam, UK), and rabbit anti-CK12 monoclonal (1:50; ab185627; Abcam, UK) primary antibodies at 4 °C overnight. ..



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    Fig. 3. (A) Agarose gel electropherogram of amplification products (passage 1). Lane 1: DNA ladder (M), lanes 2–3: KRT3 (101 bp), lanes 4–5: KRT12 (190 bp), lane 6: DNA ladder (M), lanes 7–8: TP63 (96 bp), lanes 9–10: ABCG2 (144 bp), lanes 11–12: ACTβ (131 bp), lane 13: DNA ladder (M). NC, negative control. (B) Visualization of lack of expression of CK3, and CK12 and expression of positive markers <t>p63</t> and ABCG2, in LSCs cultured in a medium supplemented with H/I/E. FITC-conjugated specific antibodies (green); DAPI- stained cell nuclei (blue). IC, isotype control. Scale bars = 15 μm.
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    Cell appearance as found by live cell and immunofluorescence imaging. (A) Live cell staining (CytoCalcein Violet 450, blue). Plasma membranes were visualized using CellMask™ Plasma Membrane Deep Red staining (red). (B) Immunocytochemistry for both stemness marker <t>p63α</t> (green) and epithelial cell marker keratin 7 (red). Cell nuclei were counterstained with DAPI (blue). Note the smaller nuclei and cells in fibrin gel culture compared to PDLLA membrane culture.
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    (A) Schematic representation illustrating the treatment paradigm with 4NQO or DMSO in 38-week-old mice. (B) Cross-sections displaying the dorsal part of the tongue labelled with the pan-epithelial marker K14 (cyan) in DMSO-treated (left panels) and 4NQO-treated mice (right panels). Unrecombined tissue expressed mTomato (red). (*) and (**) Indicate magnified area in the corresponding regions of the overview. (C) Cross-sections displaying the dorsal part of the tongue labelled with the keratinocytes marker <t>P63</t> (cyan) in DMSO-treated (left panels) vs 4NQO-treated (right panels) conditions. Unrecombined tissue expressed mTomato (red). (*) and (**) Indicate magnified areas in the corresponding regions of the overview. (D) Cross-sections displaying the dorsal part of the tongue labelled with the suppressor of transcription SNAIL (cyan) in DMSO-treated (left panel) and 4NQO-treated condition (right panels). (*) Indicates magnified area in the corresponding region of the overview. White arrows indicate Snail+ cells. Nuclear counterstaining with DAPI (blue). (E) Cross-sections displaying the dorsal part of the tongue stained for the mesenchymal marker Vimentin and the epithelial marker E-Cadherin in DMSO-treated (left panel) and 4NQO-treated (right panels) conditions. (*) and (**) Indicate magnified areas in the corresponding regions of the overview. White arrows indicate single positivity for Vimentin. Yellow arrows indicate double positivity for Vimentin and E-cadherin. Nuclear counterstaining with DAPI (blue). (*) Indicates magnified area in the corresponding region of the overview. Scale bars in overviews 100mm. Scale bars in magnification 50mm.
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    Image Search Results


    Fig. 3. (A) Agarose gel electropherogram of amplification products (passage 1). Lane 1: DNA ladder (M), lanes 2–3: KRT3 (101 bp), lanes 4–5: KRT12 (190 bp), lane 6: DNA ladder (M), lanes 7–8: TP63 (96 bp), lanes 9–10: ABCG2 (144 bp), lanes 11–12: ACTβ (131 bp), lane 13: DNA ladder (M). NC, negative control. (B) Visualization of lack of expression of CK3, and CK12 and expression of positive markers p63 and ABCG2, in LSCs cultured in a medium supplemented with H/I/E. FITC-conjugated specific antibodies (green); DAPI- stained cell nuclei (blue). IC, isotype control. Scale bars = 15 μm.

    Journal: Scientific reports

    Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells.

    doi: 10.1038/s41598-025-96919-2

    Figure Lengend Snippet: Fig. 3. (A) Agarose gel electropherogram of amplification products (passage 1). Lane 1: DNA ladder (M), lanes 2–3: KRT3 (101 bp), lanes 4–5: KRT12 (190 bp), lane 6: DNA ladder (M), lanes 7–8: TP63 (96 bp), lanes 9–10: ABCG2 (144 bp), lanes 11–12: ACTβ (131 bp), lane 13: DNA ladder (M). NC, negative control. (B) Visualization of lack of expression of CK3, and CK12 and expression of positive markers p63 and ABCG2, in LSCs cultured in a medium supplemented with H/I/E. FITC-conjugated specific antibodies (green); DAPI- stained cell nuclei (blue). IC, isotype control. Scale bars = 15 μm.

    Article Snippet: After rinsing with PBS, the cells were incubated with blocking buffer for 1 h, rinsed again, and labelled with rabbit anti-ABCG2 polyclonal (1:50; 27286-AP; Proteintech, USA), rabbit anti-p63 polyclonal (1:50; 12143-1-AP; Proteintech, USA), mouse anti-CK3 monoclonal (1:50; ab68260; Abcam, UK), and rabbit anti-CK12 monoclonal (1:50; ab185627; Abcam, UK) primary antibodies at 4 °C overnight.

    Techniques: Agarose Gel Electrophoresis, Amplification, Negative Control, Expressing, Cell Culture, Staining, Control

    Fig. 5. Squamous cell carcinoma-p63 expression –Strong (DAB,

    Journal: Journal of Veterinary and Animal Sciences

    Article Title: Pathology and evaluation of immunohistochemical expression of tumour protein 63 (p63) in corneal squamous cell carcinoma in a dog

    doi: 10.51966/jvas.2025.56.1.196-199

    Figure Lengend Snippet: Fig. 5. Squamous cell carcinoma-p63 expression –Strong (DAB,

    Article Snippet: The sections were tested with rabbit polyclonal anti-p63 antibody (1:50, Elabscience Biotechnology, USA) and was incubated overnight.

    Techniques: Expressing

    Journal: Nature Communications

    Article Title: Rosiglitazone and trametinib exhibit potent anti-tumor activity in a mouse model of muscle invasive bladder cancer

    doi: 10.1038/s41467-024-50678-2

    Figure Lengend Snippet:

    Article Snippet: Rabbit Polyclonal Anti-p63 , GeneTex , Cat#GTX102425 , N/A , 1:300.

    Techniques:

    Cell appearance as found by live cell and immunofluorescence imaging. (A) Live cell staining (CytoCalcein Violet 450, blue). Plasma membranes were visualized using CellMask™ Plasma Membrane Deep Red staining (red). (B) Immunocytochemistry for both stemness marker p63α (green) and epithelial cell marker keratin 7 (red). Cell nuclei were counterstained with DAPI (blue). Note the smaller nuclei and cells in fibrin gel culture compared to PDLLA membrane culture.

    Journal: Heliyon

    Article Title: Electrospun poly( l -lactide- co - dl -lactide) nanofibrous scaffold as substrate for ex vivo limbal epithelial cell cultivation

    doi: 10.1016/j.heliyon.2024.e30970

    Figure Lengend Snippet: Cell appearance as found by live cell and immunofluorescence imaging. (A) Live cell staining (CytoCalcein Violet 450, blue). Plasma membranes were visualized using CellMask™ Plasma Membrane Deep Red staining (red). (B) Immunocytochemistry for both stemness marker p63α (green) and epithelial cell marker keratin 7 (red). Cell nuclei were counterstained with DAPI (blue). Note the smaller nuclei and cells in fibrin gel culture compared to PDLLA membrane culture.

    Article Snippet: Incubation with anti-p63α rabbit polyclonal antibody (#4892S, Cell Signalling, Danvers, MA, USA) and anti-keratin 7 mouse monoclonal antibody (#OV-TL-12/30, Zeta Corporation, Arcadia, CA, USA) at a dilution of 1/250 was carried out at 4 °C overnight.

    Techniques: Immunofluorescence, Imaging, Staining, Clinical Proteomics, Membrane, Immunocytochemistry, Marker

    (A) Schematic representation illustrating the treatment paradigm with 4NQO or DMSO in 38-week-old mice. (B) Cross-sections displaying the dorsal part of the tongue labelled with the pan-epithelial marker K14 (cyan) in DMSO-treated (left panels) and 4NQO-treated mice (right panels). Unrecombined tissue expressed mTomato (red). (*) and (**) Indicate magnified area in the corresponding regions of the overview. (C) Cross-sections displaying the dorsal part of the tongue labelled with the keratinocytes marker P63 (cyan) in DMSO-treated (left panels) vs 4NQO-treated (right panels) conditions. Unrecombined tissue expressed mTomato (red). (*) and (**) Indicate magnified areas in the corresponding regions of the overview. (D) Cross-sections displaying the dorsal part of the tongue labelled with the suppressor of transcription SNAIL (cyan) in DMSO-treated (left panel) and 4NQO-treated condition (right panels). (*) Indicates magnified area in the corresponding region of the overview. White arrows indicate Snail+ cells. Nuclear counterstaining with DAPI (blue). (E) Cross-sections displaying the dorsal part of the tongue stained for the mesenchymal marker Vimentin and the epithelial marker E-Cadherin in DMSO-treated (left panel) and 4NQO-treated (right panels) conditions. (*) and (**) Indicate magnified areas in the corresponding regions of the overview. White arrows indicate single positivity for Vimentin. Yellow arrows indicate double positivity for Vimentin and E-cadherin. Nuclear counterstaining with DAPI (blue). (*) Indicates magnified area in the corresponding region of the overview. Scale bars in overviews 100mm. Scale bars in magnification 50mm.

    Journal: bioRxiv

    Article Title: The Notch1/Delta-like-4 axis is crucial for the initiation and progression of oral squamous cell carcinoma

    doi: 10.1101/2024.01.21.576524

    Figure Lengend Snippet: (A) Schematic representation illustrating the treatment paradigm with 4NQO or DMSO in 38-week-old mice. (B) Cross-sections displaying the dorsal part of the tongue labelled with the pan-epithelial marker K14 (cyan) in DMSO-treated (left panels) and 4NQO-treated mice (right panels). Unrecombined tissue expressed mTomato (red). (*) and (**) Indicate magnified area in the corresponding regions of the overview. (C) Cross-sections displaying the dorsal part of the tongue labelled with the keratinocytes marker P63 (cyan) in DMSO-treated (left panels) vs 4NQO-treated (right panels) conditions. Unrecombined tissue expressed mTomato (red). (*) and (**) Indicate magnified areas in the corresponding regions of the overview. (D) Cross-sections displaying the dorsal part of the tongue labelled with the suppressor of transcription SNAIL (cyan) in DMSO-treated (left panel) and 4NQO-treated condition (right panels). (*) Indicates magnified area in the corresponding region of the overview. White arrows indicate Snail+ cells. Nuclear counterstaining with DAPI (blue). (E) Cross-sections displaying the dorsal part of the tongue stained for the mesenchymal marker Vimentin and the epithelial marker E-Cadherin in DMSO-treated (left panel) and 4NQO-treated (right panels) conditions. (*) and (**) Indicate magnified areas in the corresponding regions of the overview. White arrows indicate single positivity for Vimentin. Yellow arrows indicate double positivity for Vimentin and E-cadherin. Nuclear counterstaining with DAPI (blue). (*) Indicates magnified area in the corresponding region of the overview. Scale bars in overviews 100mm. Scale bars in magnification 50mm.

    Article Snippet: Used primary antibodies: polyclonal rabbit anti-Keratin14 (1:100; Poly19053, Biolegend), polyclonal goat anti-E-Cadherin (1:200, AF748, R&D Systems), polyclonal goat anti-GFP (1:100; ab6673; Abcam), polyclonal rabbit anti-DLL4 (1:100; ab7280; Abcam), polyclonal goat anti-DLL4 (1:50; AF1389; R&D) polyclonal rabbit anti-Jag1 (1:100; ab7771; Abcam), polyclonal rabbit anti-Keratin10 (1:100; PRB-159P; Biolegend), polyclonal Rabbit anti-Keratin5 (dilution 1:100; ab64081; Abcam) polyclonal rabbit anti-Notch1 (D1E11) (dilution 1:100; 3608S; Cell Signaling), polyclonal rabbit anti-Runx1 (dilution 1:100; HPA004176; Atlas Antibodies), polyclonal rabbit anti-p63 (dilution 1:100; ab53039; Abcam), monoclonal rabbit anti-Vimentin (dilution 1:100; ab92547; Abcam), mouse anti-Snail (1:100, #14-9859-80 Invitrogen).

    Techniques: Marker, Staining

    (A) Histological examination of human squamous cell carcinoma tissue. Hematoxylin/Eosin staining is used to identify aberrant histology in two representative patients (Patient#1 left panel, Patient#2 right panel). Black arrows indicate aberrant features characteristics of squamous cell carcinoma. Expression of Jagged1 and Dll4 is shown in magnified areas (*: Patient#1; **: Patient#2). Scale bar in magnification: 100mm. Scale bar in overview: 1mm. (B) qRT-PCR on SCC25 human cell line treated with DMSO (control) or the Notch-blocker (CB103). CB103-treatment significantly affects Dll4 expression with minimal changes in Jagged1 expression. Blockage of Notch-specific transcriptional control importantly reduces the expression of the undifferentiated markers Sox2 and P63 . C) Scratch assay on SCC25 cells treated with DMSO or CB103. Scale bar 100mm. (D) Quantification of cell-free zone in the scratch assay. Each condition was normalized with the cell-free scratched area on day0. Error bars indicate standard error. **=p<0.01; ***=p<0.001; ****=p<0.0001 E) Representative images for actin filaments and focal adhesions immunofluorescence. Green staining: phalloidin. Red staining: Vinculin. Blue staining: DAPI. Scale bar 10mm. (F) Quantification of the number of focal adhesion complexes in each cell. ***=p<0.001 (G) Time lapse analyses on SCC25 cells treated with DMSO or CB103. Single snapshots for representative footages over 2h25 recording. Related to Movie 2 and Movie 3. Scale bar 50mm. H) Quantification of displacement length for each cell via Imaris software (spot tracking). ***=p<0.001

    Journal: bioRxiv

    Article Title: The Notch1/Delta-like-4 axis is crucial for the initiation and progression of oral squamous cell carcinoma

    doi: 10.1101/2024.01.21.576524

    Figure Lengend Snippet: (A) Histological examination of human squamous cell carcinoma tissue. Hematoxylin/Eosin staining is used to identify aberrant histology in two representative patients (Patient#1 left panel, Patient#2 right panel). Black arrows indicate aberrant features characteristics of squamous cell carcinoma. Expression of Jagged1 and Dll4 is shown in magnified areas (*: Patient#1; **: Patient#2). Scale bar in magnification: 100mm. Scale bar in overview: 1mm. (B) qRT-PCR on SCC25 human cell line treated with DMSO (control) or the Notch-blocker (CB103). CB103-treatment significantly affects Dll4 expression with minimal changes in Jagged1 expression. Blockage of Notch-specific transcriptional control importantly reduces the expression of the undifferentiated markers Sox2 and P63 . C) Scratch assay on SCC25 cells treated with DMSO or CB103. Scale bar 100mm. (D) Quantification of cell-free zone in the scratch assay. Each condition was normalized with the cell-free scratched area on day0. Error bars indicate standard error. **=p<0.01; ***=p<0.001; ****=p<0.0001 E) Representative images for actin filaments and focal adhesions immunofluorescence. Green staining: phalloidin. Red staining: Vinculin. Blue staining: DAPI. Scale bar 10mm. (F) Quantification of the number of focal adhesion complexes in each cell. ***=p<0.001 (G) Time lapse analyses on SCC25 cells treated with DMSO or CB103. Single snapshots for representative footages over 2h25 recording. Related to Movie 2 and Movie 3. Scale bar 50mm. H) Quantification of displacement length for each cell via Imaris software (spot tracking). ***=p<0.001

    Article Snippet: Used primary antibodies: polyclonal rabbit anti-Keratin14 (1:100; Poly19053, Biolegend), polyclonal goat anti-E-Cadherin (1:200, AF748, R&D Systems), polyclonal goat anti-GFP (1:100; ab6673; Abcam), polyclonal rabbit anti-DLL4 (1:100; ab7280; Abcam), polyclonal goat anti-DLL4 (1:50; AF1389; R&D) polyclonal rabbit anti-Jag1 (1:100; ab7771; Abcam), polyclonal rabbit anti-Keratin10 (1:100; PRB-159P; Biolegend), polyclonal Rabbit anti-Keratin5 (dilution 1:100; ab64081; Abcam) polyclonal rabbit anti-Notch1 (D1E11) (dilution 1:100; 3608S; Cell Signaling), polyclonal rabbit anti-Runx1 (dilution 1:100; HPA004176; Atlas Antibodies), polyclonal rabbit anti-p63 (dilution 1:100; ab53039; Abcam), monoclonal rabbit anti-Vimentin (dilution 1:100; ab92547; Abcam), mouse anti-Snail (1:100, #14-9859-80 Invitrogen).

    Techniques: Staining, Expressing, Quantitative RT-PCR, Wound Healing Assay, Immunofluorescence, Software