rabbit anti p63 polyclonal (Proteintech)
Structured Review

Rabbit Anti P63 Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+p63+polyclonal/p63+Antibody/pm40210649-99-26-31
Average 95 stars, based on 62 article reviews
Images
1) Product Images from "Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells."
Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells.
Journal: Scientific reports
doi: 10.1038/s41598-025-96919-2
Figure Legend Snippet: Fig. 3. (A) Agarose gel electropherogram of amplification products (passage 1). Lane 1: DNA ladder (M), lanes 2–3: KRT3 (101 bp), lanes 4–5: KRT12 (190 bp), lane 6: DNA ladder (M), lanes 7–8: TP63 (96 bp), lanes 9–10: ABCG2 (144 bp), lanes 11–12: ACTβ (131 bp), lane 13: DNA ladder (M). NC, negative control. (B) Visualization of lack of expression of CK3, and CK12 and expression of positive markers p63 and ABCG2, in LSCs cultured in a medium supplemented with H/I/E. FITC-conjugated specific antibodies (green); DAPI- stained cell nuclei (blue). IC, isotype control. Scale bars = 15 μm.
Techniques Used: Agarose Gel Electrophoresis, Amplification, Negative Control, Expressing, Cell Culture, Staining, Control
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Incubation:Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells Article Snippet: LSCs after first passage were cultured on cover slips, fixed with 4% formaldehyde, and permeabilized with 0.5% Triton X-100 solution (Sigma-Aldrich, USA) and Tween 20 (Sigma-Aldrich, USA). .. After rinsing with PBS, the cells were incubated with blocking buffer for 1 h, rinsed again, and labelled with rabbit anti-ABCG2 polyclonal (1:50; 27286-AP; Proteintech, USA), Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells. Article Snippet: LSCs after first passage were cultured on cover slips, fixed with 4% formaldehyde, and permeabilized with 0.5% Triton X-100 solution (Sigma-Aldrich, USA) and Tween 20 (Sigma-Aldrich, USA). .. After rinsing with PBS, the cells were incubated with blocking buffer for 1 h, rinsed again, and labelled with rabbit anti-ABCG2 polyclonal (1:50; 27286-AP; Proteintech, USA), Blocking Assay:Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells Article Snippet: LSCs after first passage were cultured on cover slips, fixed with 4% formaldehyde, and permeabilized with 0.5% Triton X-100 solution (Sigma-Aldrich, USA) and Tween 20 (Sigma-Aldrich, USA). .. After rinsing with PBS, the cells were incubated with blocking buffer for 1 h, rinsed again, and labelled with rabbit anti-ABCG2 polyclonal (1:50; 27286-AP; Proteintech, USA), Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells. Article Snippet: LSCs after first passage were cultured on cover slips, fixed with 4% formaldehyde, and permeabilized with 0.5% Triton X-100 solution (Sigma-Aldrich, USA) and Tween 20 (Sigma-Aldrich, USA). .. After rinsing with PBS, the cells were incubated with blocking buffer for 1 h, rinsed again, and labelled with rabbit anti-ABCG2 polyclonal (1:50; 27286-AP; Proteintech, USA), |



