rabbit anti p63 polyclonal  (Proteintech)

Journal: Scientific reports

Article Title: Assessment of the toxic effect of benzalkonium chloride on human limbal stem cells.

doi: 10.1038/s41598-025-96919-2

Figure Lengend Snippet: Fig. 3. (A) Agarose gel electropherogram of amplification products (passage 1). Lane 1: DNA ladder (M), lanes 2–3: KRT3 (101 bp), lanes 4–5: KRT12 (190 bp), lane 6: DNA ladder (M), lanes 7–8: TP63 (96 bp), lanes 9–10: ABCG2 (144 bp), lanes 11–12: ACTβ (131 bp), lane 13: DNA ladder (M). NC, negative control. (B) Visualization of lack of expression of CK3, and CK12 and expression of positive markers p63 and ABCG2, in LSCs cultured in a medium supplemented with H/I/E. FITC-conjugated specific antibodies (green); DAPI- stained cell nuclei (blue). IC, isotype control. Scale bars = 15 μm.

Article Snippet: After rinsing with PBS, the cells were incubated with blocking buffer for 1 h, rinsed again, and labelled with rabbit anti-ABCG2 polyclonal (1:50; 27286-AP; Proteintech, USA), rabbit anti-p63 polyclonal (1:50; 12143-1-AP; Proteintech, USA), mouse anti-CK3 monoclonal (1:50; ab68260; Abcam, UK), and rabbit anti-CK12 monoclonal (1:50; ab185627; Abcam, UK) primary antibodies at 4 °C overnight.

Techniques: Agarose Gel Electrophoresis, Amplification, Negative Control, Expressing, Cell Culture, Staining, Control

Journal: Nature Communications

Article Title: Rosiglitazone and trametinib exhibit potent anti-tumor activity in a mouse model of muscle invasive bladder cancer

doi: 10.1038/s41467-024-50678-2

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal Anti-p63 , GeneTex , Cat#GTX102425 , N/A , 1:300.

Techniques:

Journal: Heliyon

Article Title: Electrospun poly( l -lactide- co - dl -lactide) nanofibrous scaffold as substrate for ex vivo limbal epithelial cell cultivation

doi: 10.1016/j.heliyon.2024.e30970

Figure Lengend Snippet: Cell appearance as found by live cell and immunofluorescence imaging. (A) Live cell staining (CytoCalcein Violet 450, blue). Plasma membranes were visualized using CellMask™ Plasma Membrane Deep Red staining (red). (B) Immunocytochemistry for both stemness marker p63α (green) and epithelial cell marker keratin 7 (red). Cell nuclei were counterstained with DAPI (blue). Note the smaller nuclei and cells in fibrin gel culture compared to PDLLA membrane culture.

Article Snippet: Incubation with anti-p63α rabbit polyclonal antibody (#4892S, Cell Signalling, Danvers, MA, USA) and anti-keratin 7 mouse monoclonal antibody (#OV-TL-12/30, Zeta Corporation, Arcadia, CA, USA) at a dilution of 1/250 was carried out at 4 °C overnight.

Techniques: Immunofluorescence, Imaging, Staining, Clinical Proteomics, Membrane, Immunocytochemistry, Marker

Journal: bioRxiv

Article Title: The Notch1/Delta-like-4 axis is crucial for the initiation and progression of oral squamous cell carcinoma

doi: 10.1101/2024.01.21.576524

Figure Lengend Snippet: (A) Schematic representation illustrating the treatment paradigm with 4NQO or DMSO in 38-week-old mice. (B) Cross-sections displaying the dorsal part of the tongue labelled with the pan-epithelial marker K14 (cyan) in DMSO-treated (left panels) and 4NQO-treated mice (right panels). Unrecombined tissue expressed mTomato (red). (*) and (**) Indicate magnified area in the corresponding regions of the overview. (C) Cross-sections displaying the dorsal part of the tongue labelled with the keratinocytes marker P63 (cyan) in DMSO-treated (left panels) vs 4NQO-treated (right panels) conditions. Unrecombined tissue expressed mTomato (red). (*) and (**) Indicate magnified areas in the corresponding regions of the overview. (D) Cross-sections displaying the dorsal part of the tongue labelled with the suppressor of transcription SNAIL (cyan) in DMSO-treated (left panel) and 4NQO-treated condition (right panels). (*) Indicates magnified area in the corresponding region of the overview. White arrows indicate Snail+ cells. Nuclear counterstaining with DAPI (blue). (E) Cross-sections displaying the dorsal part of the tongue stained for the mesenchymal marker Vimentin and the epithelial marker E-Cadherin in DMSO-treated (left panel) and 4NQO-treated (right panels) conditions. (*) and (**) Indicate magnified areas in the corresponding regions of the overview. White arrows indicate single positivity for Vimentin. Yellow arrows indicate double positivity for Vimentin and E-cadherin. Nuclear counterstaining with DAPI (blue). (*) Indicates magnified area in the corresponding region of the overview. Scale bars in overviews 100mm. Scale bars in magnification 50mm.

Article Snippet: Used primary antibodies: polyclonal rabbit anti-Keratin14 (1:100; Poly19053, Biolegend), polyclonal goat anti-E-Cadherin (1:200, AF748, R&D Systems), polyclonal goat anti-GFP (1:100; ab6673; Abcam), polyclonal rabbit anti-DLL4 (1:100; ab7280; Abcam), polyclonal goat anti-DLL4 (1:50; AF1389; R&D) polyclonal rabbit anti-Jag1 (1:100; ab7771; Abcam), polyclonal rabbit anti-Keratin10 (1:100; PRB-159P; Biolegend), polyclonal Rabbit anti-Keratin5 (dilution 1:100; ab64081; Abcam) polyclonal rabbit anti-Notch1 (D1E11) (dilution 1:100; 3608S; Cell Signaling), polyclonal rabbit anti-Runx1 (dilution 1:100; HPA004176; Atlas Antibodies), polyclonal rabbit anti-p63 (dilution 1:100; ab53039; Abcam), monoclonal rabbit anti-Vimentin (dilution 1:100; ab92547; Abcam), mouse anti-Snail (1:100, #14-9859-80 Invitrogen).

Techniques: Marker, Staining

Journal: bioRxiv

Article Title: The Notch1/Delta-like-4 axis is crucial for the initiation and progression of oral squamous cell carcinoma

doi: 10.1101/2024.01.21.576524

Figure Lengend Snippet: (A) Histological examination of human squamous cell carcinoma tissue. Hematoxylin/Eosin staining is used to identify aberrant histology in two representative patients (Patient#1 left panel, Patient#2 right panel). Black arrows indicate aberrant features characteristics of squamous cell carcinoma. Expression of Jagged1 and Dll4 is shown in magnified areas (*: Patient#1; **: Patient#2). Scale bar in magnification: 100mm. Scale bar in overview: 1mm. (B) qRT-PCR on SCC25 human cell line treated with DMSO (control) or the Notch-blocker (CB103). CB103-treatment significantly affects Dll4 expression with minimal changes in Jagged1 expression. Blockage of Notch-specific transcriptional control importantly reduces the expression of the undifferentiated markers Sox2 and P63 . C) Scratch assay on SCC25 cells treated with DMSO or CB103. Scale bar 100mm. (D) Quantification of cell-free zone in the scratch assay. Each condition was normalized with the cell-free scratched area on day0. Error bars indicate standard error. **=p<0.01; ***=p<0.001; ****=p<0.0001 E) Representative images for actin filaments and focal adhesions immunofluorescence. Green staining: phalloidin. Red staining: Vinculin. Blue staining: DAPI. Scale bar 10mm. (F) Quantification of the number of focal adhesion complexes in each cell. ***=p<0.001 (G) Time lapse analyses on SCC25 cells treated with DMSO or CB103. Single snapshots for representative footages over 2h25 recording. Related to Movie 2 and Movie 3. Scale bar 50mm. H) Quantification of displacement length for each cell via Imaris software (spot tracking). ***=p<0.001

Article Snippet: Used primary antibodies: polyclonal rabbit anti-Keratin14 (1:100; Poly19053, Biolegend), polyclonal goat anti-E-Cadherin (1:200, AF748, R&D Systems), polyclonal goat anti-GFP (1:100; ab6673; Abcam), polyclonal rabbit anti-DLL4 (1:100; ab7280; Abcam), polyclonal goat anti-DLL4 (1:50; AF1389; R&D) polyclonal rabbit anti-Jag1 (1:100; ab7771; Abcam), polyclonal rabbit anti-Keratin10 (1:100; PRB-159P; Biolegend), polyclonal Rabbit anti-Keratin5 (dilution 1:100; ab64081; Abcam) polyclonal rabbit anti-Notch1 (D1E11) (dilution 1:100; 3608S; Cell Signaling), polyclonal rabbit anti-Runx1 (dilution 1:100; HPA004176; Atlas Antibodies), polyclonal rabbit anti-p63 (dilution 1:100; ab53039; Abcam), monoclonal rabbit anti-Vimentin (dilution 1:100; ab92547; Abcam), mouse anti-Snail (1:100, #14-9859-80 Invitrogen).

Techniques: Staining, Expressing, Quantitative RT-PCR, Wound Healing Assay, Immunofluorescence, Software

Journal: bioRxiv

Article Title: Heterogeneous potential of human airway basal cells: Holoclone-stem cell identification in a clinical-grade system

doi: 10.1101/2023.12.28.573530

Figure Lengend Snippet: (A) IF staining of AT (left) and AB (right) cultures at four different time points of airway epithelial cell lifespan (representative images of n = 3 AT and n = 3 AB primary human independent strains). Scale bars, 50 μm. (B) WB analysis on total cell extracts from six expansion passages (AT2 strain) representative of five consecutive lifespan intervals, immunostained with indicated antibodies. Experiment conducted on n = 3 AT strains (n = 5 technical replicates) and n = 3 AB strains (n = 4 technical replicates). (C) Histograms showing the quantification of the expression levels from top left to bottom right of CK14, Involucrin, p63α, BMI1 (all normalized per GAPDH) and SOX2 (normalized per Vinculin). Average and SD of n = 3 AT and n = 3 AB displayed per each time range (see “Star Methods”). Independent strains are indicated with different shapes. Unpaired, biparametric, two-tailed t test *p < 0.05.; ** p < 0.01 and *** p < 0.001.

Article Snippet: The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal anti-GAPDH (1:10.000, Abcam), mouse monoclonal anti-Vinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), mouse monoclonal anti-Actin (1:5000, Abcam).

Techniques: Staining, Expressing, Two Tailed Test

Journal: bioRxiv

Article Title: Heterogeneous potential of human airway basal cells: Holoclone-stem cell identification in a clinical-grade system

doi: 10.1101/2023.12.28.573530

Figure Lengend Snippet: (A) Violin plot showing the size measured in mm of AT (left) and AB (right) different clones. AT analysed clones: H, n = 36; EM, n = 86; IM, n = 112; LM, n = 56; P, n = 45 belonging to n = 3 independent AT strains; AB analysed clones: H, n = 35; EM, n = 44; IM, n = 52; LM, n = 13; P, n = 9 belonging to n = 2 independent AB strains. Dots represent single clones. Median, first and third quartiles are displayed. (B) WB analysis on total cell extracts from the progeny of AT2 clones (H, n = 3; EM, n = 2; IM, n = 1; LM, n = 2) immunostained with indicated antibodies (image representative of n = 2 analysis conducted on independent clones). Due to their very limited residual proliferative potential, the amount of material collected by AT2 paraclone’s progenies was not sufficient to carry out a reliable WB analysis. (C) From left to right, histograms showing the quantification of the expression levels of p63α, BMI1 (normalized per GAPDH) and SOX2 (normalized per GAPDH in AT and Actin in AB). Average and SD displayed per each clonal category (see “Star Methods”). AT2 analysed clones: H, n = 3; EM, n = 5; IM, n = 5; LM, n = 3. AB1 analysed clones: H, n = 3; EM, n = 2; IM, n = 3; LM, n = 3. Unpaired, biparametric, two-tailed t test *p < 0.05.; ** p < 0.01; *** p < 0.001 and **** p < 0.0001.

Article Snippet: The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal anti-GAPDH (1:10.000, Abcam), mouse monoclonal anti-Vinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), mouse monoclonal anti-Actin (1:5000, Abcam).

Techniques: Clone Assay, Expressing, Two Tailed Test